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Propagation of Ornamental Plants
11(3): 149-155, 2011
IN VITRO PROPAGATION OF CYMBIDIUM NANUTUM Y. S. WU ET S. C. CHEN
Zhongming Fang1,2, Weiting Huang1,2, Songjun Zeng1,2*, Kunlin Wu1, Zhilin Chen1, Mingyong Zhang1, and Jun Duan1
1 Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China, *Fax: 86-20-37252831, *E-mail: zengsongjun@scib.ac.cn 2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
Abstract
An efficient micropropagation system has been developed for Cymbidium nanutum Y. S. Wu et S. C. Chen using immature seeds. Seed germination frequency was 45% after 60 days culture and protocorms subsequently formed rhizomes after 90 days culture on half strength Murashige and Skoog (½ MS) medium with 0.5 mg l-1 NAA, 30 g l-1 sucrose, 1 g l-1 activated charcoal, and 100 ml l-1 coconut milk. The highest average proliferation rate was 4.4 and was obtained after 60 days of culture on half strength MS medium containing 2.0 mg l-1 NAA, 0.5 mg l-1 BAP, 10 g l-1 sucrose, and 1 g l-1 activated charcoal. Approximately 1 cm long rhizomes produced an average of 3.7 shoot after 50 days of culture on half strength MS differentiation medium supplemented with 0.1 mg l-1 TDZ, 0.25 mg l-1 NAA, 20 g l-1 sucrose, and 1.0 g l-1 citric acid. Hyponex medium (1.5 g l-1 Hyponex1 and 1.5 g l-1 Hyponex2) supplemented with 0.5 mg l-1 NAA, 30 g l-1 sucrose, 2.0 g l-1 activated charcoal, 100 ml l-1 coconut milk and 100 g l-1 banana homogenate was best for shoot bud growth and rooting induction (69.7% rooting). The plantlets survival rate was 60% after they were transfered to pots filled with a 1 : 1 : 1 (v : v : v) sand : sieved peat : perlite in greenhouse 30 days after the transferring.
Key words: bud, Cymbidium nanutum, organogenesis, rhizome, tissue culture
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