ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(1): 23-34, 2005

PHOTOAUTOTROPHIC (SUGAR-FREE MEDIUM) MICROPROPAGATION SYSTEMS FOR LARGE-SCALE COMMERCIALIZATION

Toyoki Kozai1*, Yulan Xiao¹, Quynh T. Nguyen², Fawzia Afreen¹ and Sayed M. A. Zobayed¹

¹Faculty of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan, *Fax: + 81 47 308 8841, *e-mail: kozai@faculty.chiba-u.jp
²Institute of Tropical Biology, NCST-VN, 1Mac Dinh Chi Street, Dist. 1. Hochiminh City, Vietnam


Abstract
Our recent research has revealed that most chlorophyllous explants/plants in vitro including cotyledonary stage somatic embryos have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to the poor photosynthetic ability, but because of the low CO2 concentration in the air-tight culture vessel during the photoperiod. Furthermore, we have shown that the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. Recently, a photoautotrophic or sugar-free medium micropropagation system (PAM) consisting of five large culture vessels (volume: 120 l each) with a forced ventilation unit for supplying CO2-enriched air was developed and applied for the commercial production of calla lily (Zantedeschia elliottiana) and China fir (Cunningghamia lanceolata) plantlets in China. The culture period of calla lily plantlets in the PAM was reduced by 50%, compared with that in a conventional, photomixotrophic micropropagation system (PMM) using small vessels each containing a sugar-containing medium. Percent survival ex vitro of calla lily plantlets from the PAM was 95%, while that from the PMM was 60%. The production cost of calla lily in the PAM was reduced by about 40%, compared with that in the PMM, and the initial investment per plantlet for the PAM was about 10% lower than that for the PMM. The sales price of ex vitro acclimatized calla lily plantlet was increased by 25% due to its higher quality, compared with plantlets produced in the PMM.

Key words: CO2 enrichment, Cunningghamia lanceolata, forced ventilation, natural ventilation, production cost, porous supporting material, sugar-free medium, Zantedeschia elliottiana.



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