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Propagation of Ornamental Plants
23(4): 91-98, 2023
MICROPROPAGATION OF LONG-LEAVED PAPERBARK (MELALEUCA LEUCADENDRA (L.) L.)
Pham Thi Diem Thi1, Nguyen Thi Nguyen Man2, Nguyen Thi Khanh Quynh2, Trieu Thy Hoa3, Pham Mai Thu Thuy1, and Hoang Tan Quang1*
1 Institute of Biotechnology, Hue University, Nguyen Dinh Tu str., 49000 Thua Thien Hue, Vietnam, *E-mail: htquang@hueuni.edu.vn
2 QueenLabs Biotechnology One Member Company, 27 Ngu Binh str, 49000 Thua Thien Hue, Vietnam
3 Department of Natural Sciences and Technology, Quang Nam University, 102 Hung Vuong str, 51000 Quang Nam, Vietnam
This study aimed to develop a rapid method for micropropagation of Melaleuca leucadendra. Nodal explants were washed and decontaminated with 0.1% HgCl2 for 16 min with the highest survival rate of 75.6%. The cultivation of nodal segments in basal WPM (Woody Plant Medium) supplemented with 30 g l-1 sucrose, 9.0 g l-1 agar, and 0.5 mg l-1 BAP resulted in the highest axillary shoot formation (3.0) after a month. The full-strenght WPM with 1.5 mg l-1 BAP and 0.1 mg l-1 TDZ proved effective for shoot multiplication (11.4 shoots), while the optimal for rooting was half-strength WPM with 1.0 mg l-1 NAA (100% rooting with 4.3 roots).
Key words: in vitro culture, rooting, shoot multiplication, WPM
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