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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
21(4): 116-122, 2021

THE ROLE OF PYROLIGNEOUS ACID FOR IN VITRO PROPAGATION
OF PHORMIUM TENAX ʽVARIEGATUMʼ (J. R. FORST & G. FORST)

Güldane Aslı Turp1*, Sinan Mehmet Turp2, and Saim Ozdemir1

1 Department of Environmental Engineering, Faculty of Engineering, Sakarya University, 1 University str., 54050 Sakarya, Turkey, *E-mail: g.asliturp@gmail.com
2 Department of Environmental Engineering, Faculty of Engineering, Bitlis Eren University, 1 University str., Rahva Campus, 13100 Bitlis, Turkey



The effect of pyroligneous acid (PA), a byproduct of woody biomass pyrolysis, was researched for both antifungal activity and growth promotion in micropropagated in vitro plantlets during the ex vitro acclimatization process. The micropropagated plantlets of Phormium tenax J. R. Forst. & G. Forst ‘Variegatum’ were transplanted on a coco peat substrate that was treated with PA. The antifungal test was conducted to assess the ability to acclimatization ex vitro. The results showed that the PA completely inhibited soil-borne fungi activity at the concentrations of 1.5, 1.5, and 2.5% of the Rhizoctonia solani (Kühn), Verticillum dahliae (Kleb.), and Fusarium oxysporum (f. sp. Vasinfectum), respectively. The PA levels were above 86% of the researched Phormium tenax ‘Variegatum’. Therefore, both survival rate of acclimatized plantlets and standard coco peat were consistent considering the PA levels. The application of PA into the potting substrate at a rate of 2% and 5% had positive effects on survival rate of ≥ 90% in terms of plant height, shoot, and root dry biomass, and seedling quality index, compared to the plants acclimatized on coco peat control. The findings demonstrated that the application of PA in potting media at specific concentrations, can suppress soil-borne pathogens by providing plant nutrients. Furthermore, the growth rate promotes and enhance the seedling growth parameters of micro-propagated plants during ex vitro acclimatization process.

Key words: acclimatization, anti-fungal activity, in vitro plantlets, phormium, pyroligneous acid



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