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Propagation of Ornamental Plants
16(3): 73-78, 2016

RESPONSE TO PEG-INDUCED HYDRIC STRESS ON IN VITRO GERMINATION OF
PROSTHECHEA VITELLINA (LINDL.) W. E. HIGGINS (ORCHIDACEAE)

Francisco Jácome-Blásquez1, Victorino Morales-Ramos2, María de Jesús Martínez-Hernández1, Gabriela Sánchez-Viveros1, and Jericó Jabín Bello-Bello2*

1 Faculty of Agricultural Sciences, University of Veracruz-Campus Xalapa, Gonzalo Aguirre Beltran circuit, 91090 Xalapa, State of Veracruz, México,
2 Postgraduate College-Campus Córdoba, km. 348 Córdoba-Veracruz Federal Highway, 94946 Amatlán de los Reyes, State of Veracruz, México, *Fax: + 52 (271) 71 6 60 00, *E-mail: jericobello@gmail.com



Hydric stress is one of the main problems facing plants as a result of climate change. The purpose of this study was to evaluate the effect of polyethylene glycol (PEG)-induced hydric stress on in vitro germination of P. vitellina. Seeds were cultivated on Murashige and Skoog semisolid medium without growth regulators and supplemented with PEG (0%, 5%, 10%, and 15%), inducing osmotic potentials of -0.42, -0.54, -0.69, and -1.02 Mpa, respectively. After 60 days of culture, germination decreased as PEG concentration increased. The treatment containing 10% PEG showed a germination of 55.33%. The highest germination rate was obtained in seeds without PEG (95.66%), while the lowest germination was 36%, with 15% PEG. The content of proline and glycine betaine in protocorms increased substantially in response to polyethylene glycol. These results suggest that P. vitellina can tolerate an osmotic potential of -0.69 Mpa.

Key words: glycine-betaine, hydric stress, orchid, proline, protocorms



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