Wisteria frutescens var. macrostachya ‘Blue Moon’ is a flowering vine native to the United States and is the hardiest of the wisteria in USDA winter hardiness zone 4a. With the increased hardiness of the species, ‘Blue Moon’ is of interest for improvement in terms of increased flowering, dwarfing, and sterility. The objective of this study was to establish a micropropagation protocol involving axillary shoot proliferation and subsequent in vitro rooting of ‘Blue Moon’ wisteria axillary shoots. A 4 x 5 factorial of four nutrient salt formulations [Driver and Kuniyuki (DKW), Long and Preece (LP), Murashige and Skoog (MS), and Woody Plant Medium (WPM)] and five concentrations (0, 2, 4, 8, 16 μM) of N⁶-benzyladenine (BA) were used to determine shoot proliferation of viable axillary shoots. With respect to propagation rate, there was a significant interaction between nutrient salt formulation and BA concentration with DKW and 4 μM BA producing the most axillary shoots per nodal segment as compared to other combinations evaluated. A 2 × 4 factorial of two auxin types (IBA and IAA) and four concentrations (0, 2.5, 5.0, and 10 μM) were used to determine subsequent axillary shoot rooting capabilities. With respect to root number, there was a significant interaction between auxin type and concentration with 10 μM IAA producing the most roots (6.1) per axillary shoot as compared to other auxin types and concentrations evaluated with 100% rooting. All rooted axillary shoots were successfully acclimatized within greenhouse conditions. Rooted shoots utilizing 10 μM IAA produced the highest quality and best rooted axillary shoots to work with.