Propagation of Ornamental Plants
17(2): 48-54, 2017
IN VITRO PROPAGATION OF CYMBIDIUM HYBRID
Doo Hwan Kim1, Kyung Won Kang2, and Iyyakkannu Sivanesan1*
1 Department of Bioresources and Food Science, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, 143-701 Seoul, Republic of Korea, *Fax: +8224503310
2 Babo Orchid Farm, Namyangju-si, Gyeonggi-do 472-831, Republic of Korea
An efficient in vitro propagation protocol was established for Cymbidium hybrid (Cymbidium ensifolium × Cymbidium ‘Enid Haupt’). Activated charcoal (AC), coconut water (CW), and α-naphthaleneacetic acid (NAA) had a significant effect on seed germination and protocorm induction. The maximum frequency seed germination (86.8%) and protocorm formation (61.8%) were obtained on Murashige and Skoog (MS) medium fortified with 0.2% CW, 0.2% AC, and 0.1 mg l-1 NAA. The highest frequency of secondary protocorm induction (96%) with a mean of 13.2 secondary protocorms was obtained on MS medium fortified with 0.2% AC, 0.3% apple juice, 3% banana pulp, 1% potato homogenate, 0.3 mg l-1 kinetin, and 0.1 mg l-1 NAA. On this medium, a maximum of 81.2% protocorms were converted to shoots. Marginal and random leaf variegation patterns were obtained in regenerated shoots. The yellowish white stripe(s) was observed on leaf margins or whole leaf. The in vitro-developed shoots were rooted best on Hyponex medium containing 0.2% AC, 0.3% apple juice, 5% banana pulp, 0.2% peptone, 1% potato homogenate, and 0.3 mg l-1 NAA. The normal and variegated plantlets were successfully acclimatized in the greenhouse.
Key words: activated charcoal, kinetin, orchid, protocorm, seed germination, variegation