ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
15(2): 63-72, 2015


Raluca Cicevan1, Mohamad Al Hassan2, Adriana Sestras1, Monica Boscaiu3*, Adrian Zaharia1, Oscar Vicente2, and Radu Sestras1

1 University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Manastur str., 400372 Cluj-Napoca, Romania
2 Institute of Plant Molecular and Cellular Biology (IBMCP, UPV-CSIC), Polytechnic University of Valencia, CPI, Building 8E, Camino de Vera s/n, 46022 Valencia, Spain
3 Mediterranean Agroforestry Institute (IAM, UPV), Polytechnic University of Valencia, CPI, Building 8E, Camino de Vera s/n, 46022 Valencia, Spain, *Fax: + 34-96-387 92 69,

Drought and salinity are the most adverse environmental factors affecting crop productivity worldwide. The increasing scarcity of fresh, high-quality irrigation water is forcing the use of alternative water sources-such as sewage, waste water or brackish water in agriculture, in general, and also in floriculture. Seed germination is the first and the most important stage in a plant’s life cycle, but it is also the most sensitive to environmental conditions, representing a bottleneck in plant development. Checking for stress tolerance at the germination stage is a reliable approach for the rapid screening of a large number of plant cultivars. The aim of the present study was to analyze the responses to osmotic and ionic stress, induced by isotonic solutions of polyethylene glycol (PEG) and NaCl, in 13 cultivars of Tagetes patula, T. tenuifolia, and T. erecta at the germination stage. Germination percentages and rates, and radicle, hypocotyls, and cotyledon lengths were determined after seven days of treatment. Responses to osmotic stress induced by PEG were similar in all selected cultivars, but significant differences were detected upon the salt treatments. In general, T. erecta responded better to salt stress than the other two species, but a large variability among cultivars within each species was observed. The most stress-tolerant cultivars of T. patula were ‘Orion’and ‘Robuszta’, and those of T. tenuifolia were ‘Luna Gold’, ‘Luna Rot’ and ‘Luna Orange’.

Key words: genotypes, osmotic stress, salt stress, Tagetes erecta, T. patula, T. tenuifolia

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