ISSN 1311-9109 Journal Content





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CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
10(4): 246-252, 2010

MICROPROPAGATION OF ALSTROEMERIA IN LIQUID MEDIUM USING
SLOW RELEASE OF MEDIUM COMPONENTS

Geert-Jan de Klerk* and Jolanda ter Brugge

WageningenUR Plant Breeding, PO Box 16, 6700 AA Wageningen, The Netherlands,
*Fax: + 31317483457; *E-mail: geertjan.deklerk@wur.nl


Abstract
Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous atmosphere of the headspace. The medium became rapidly depleted with respect to both organic and inorganic nutrients. Manual addition of extra medium ingredients during the culture enhanced growth. Extra nutrients were also successfully administered via slow-release tools. Two types of slow-release tools were used, Osmocote for inorganic nutrients and newly developed castings for sucrose. Addition of extra 6-benzylaminopurine (BAP) during the culture stimulated the outgrowth of lateral rhizomes. The extra BAP could be included in the slow-release castings for sucrose.

Key words: alstroemeria, liquid medium, nutrients, Osmocote, slow release



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