ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
10(4): 237-245, 2010

MICROPROPAGATION THROUGH SOMATIC EMBRYOGENESIS OF CYCLAMEN PERSICUM MILL. GENOTYPES FOR CUT FLOWER PRODUCTION –
FEASIBILITY STUDY

Traud Winkelmann1*, Agnieszka Ilczuk2, and Stephan Wartenberg3

1Institute of Floriculture and Woody Plant Science, Leibniz Universitaet Hannover, Herrenhaeuser str. 2, D-30419 Hannover, Germany, *Fax: + 49 511 762 3608,
*E-mail: traud.winkelmann@zier.uni-hannover.de

2
Department of Ornamental Plants, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Science, 159 Nowoursynowska str., 02-776 Warsaw, Poland

3
Saxon State Office for Environment, Agriculture and Geology, Department of Horticulture, Dresden-Pillnitz, Soebrigener str. 3a, 01326 Dresden, Germany


Abstract
The regeneration of Cyclamen persicum through somatic embryogenesis (SE) has been described by several groups for a wide spectrum of genotypes. Here, we studied this regeneration system for the first time with 30 genotypes which are valued as cut flowers. The aim of this study was to analyse the efficiency of the micropropagation through SE, to investigate the genetic fidelity and performance of the regenerants and to estimate production costs of young plants. The genotype significantly influenced the frequency of callus induction that ranged from 8 to 80% and also the frequency of calluses with differentiation of somatic embryos that ranged from 0 to 92%. Only three of the 30 genotypes were not able to regenerate somatic embryos, while plants were obtained from the remaining 27 genotypes. In total, 2,783 regenerants were transferred to the greenhouse, 2,003 (72%) of which could be acclimatized. Some genotypes were extremely uniform in terms of flower colour and flower shapes, whereas in others somaclonal variation was detected. Estimation of micropropagation cost was 2-3 € per acclimatized young plant at our research facility. If genotypes with high regeneration efficiencies are selected, plant production costs could be reduced to 0.56-0.80 € or even more.

Key words: Cyclamen, genetic fidelity, performance test, production costs, in vitro propagation, somaclonal variation



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