|
Propagation of Ornamental Plants
9(4): 216-222, 2009
ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATION
OF CHIRITA FLAVIMACULATA W. T. WANG, C. EBURNEA HANCE,
AND C. SPECIOSA KURZ.
Masaru Nakano1*, Hiroki Takagi1, Shintaro Sugawara1, Tomoka Saito1, Yusuke Watanabe1,2, Lu Yuanxue3, Guan Kaiyun3, and Toshinari Godo4
1Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata 950-2181, Japan, *Fax: + 81-25-262-6858, *E-mail: mnakano@agr.niigata-u.ac.jp
2Niigata Agricultural Research Institute, Horticultural Research Center, 177 Mano,
Seiro 957-0111, Japan
3Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Heilongtan, Kunming, Yunnan 650204, China
4Botanic Gardens of Toyama, 42 Kamikutsuwada, Fuchu-Machi, Toyama 939-2713, Japan
Abstract
Chirita flavimaculata, C. eburnea, and C. speciosa, which belong to Gesneriaceae, have high ornamental values such as beautiful foliages and showy flowers. An efficient plant regeneration system via adventitious shoot formation was established for micropropagation of these species. For all species, adventitious shoots elongating over 5 mm in length were efficiently regenerated from leaf explants on half-strength MS medium containing 0.1 mg l-1 of both NAA and BA (8.1-15.6 shoots per explant). Histological observation indicated that adventitious shoot regeneration on a medium containing 0.1 mg l-1 of both NAA and BA occurred directly from leaf epidermal cells without callus formation. Plantlets were obtained by rooting the shoots on half-strength MS medium containing 0.1 mg l-1 IBA. No variations in the ploidy level were observed in the regenerated plants of three Chirita species as indicated by flow cytometry analysis.
Key words: Chirita, flow cytometry analysis, histological observation, plant regeneration, ploidy stability, rooting
|
