Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana ‘Molly’ and breeding line ‘1998-469’. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l^-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l^-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for ‘Molly’ and 6.7-39% for breeding line ‘1998-469’. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.