Somatic embryogenesis of Gladiolus anatolicus was induced using in vitro obtained cormel segments. Embryogenic callus induction was carried out on Murashige and Skoog (MS) medium containing various concentrations of 2,4-D in dark. The best response was observed in the medium containing 4.5 μM 2,4-D. Eight weeks after culture initiation, the yellowish-pale and compact calluses were transferred to MS medium containing different concentrations of BA (0.5, 2.2, 4.5, 9.0 and 18.0 μM) for embryo induction and development. The highest number of somatic embryos (23.6 ± 3.6) was produced at the lowest concentration of BA (0.5 μM). The number of somatic embryos was significantly enhanced from 23.6 ± 3.6 up to 31.6 ± 3.8 by adding 12% sucrose to the embryonic callus culture media containing 0.5 μM BA. Somatic embryos in cotyledonarystage were transferred to MS medium supplemented with 0.5 μM BA and 2% sucrose for maturation and germination. Maximum germination rate was 50%. Application of this protocol has potential in large-scale clonal propagation of the Gladiolus anatolicus for ornamental purposes.