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Propagation of Ornamental Plants
9(2): 97-101, 2009
TISSUE CULTURE AND RAPID PROPAGATION OF CHIRITA MEDICA
D. FANG EX W. T. WANG
Jia Li1,2, Quan Xing1, Weilun Chen1, Donghong Guo1, and Lei Shi1*
1 Institute of Botany, The Chinese Academy of Science, Beijing, China 100093
2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
*Fax: + 86-010-82593616, *E-mail: shilei67@263.net
Abstract
A successful procedure to regenerate Chirita medica D. Fang ex W.T. Wang from leaf explants was developed. Culturing leaf explants on MS medium with 0.1 mg l-1 NAA and 0.1 mg l-1 BA for 8 weeks produced the highest adventitious bud induction rate of 90.3%. Half-strength MS medium with 5 g l-1 activate charcoal and 30 g l-1 sucrose was optimal for root induction which caused 100% rooting, 6.11 roots averaging 18.8 mm in length. Rooted plants, transferred to field conditions, could reach 100% survival in two months.
Key words: adventitious bud, Chirita medica, in vitro plant regeneration, leaf explants
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