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Propagation of Ornamental Plants
9(2): 90-96, 2009
ROOTING, QUANTIFICATION OF INDOLE-3-ACETIC ACID AND INDOLE-3-BUTYRIC ACID, AND IBA TRANSPORT IN GREVILLEA
Santi Krisantini1*, Margaret Johnston2, Christine Anne Beveridge3,
John Ross4, and Richard Williams2
1 Department of Agronomy and Horticulture, Bogor Agricultural University, Bogor, Indonesia 16680, *Fax: + 62-251-629 353, *E-mail: krisantini@yahoo.com.au
2 School of Land, Crop and Food Sciences, University of Queensland,
Gatton Campus, Queensland, Australia 4343
3 School of Integrative Biology, University of Queensland, St. Lucia Campus,
Queensland, Australia 4071
4School of Plant Science, The University of Tasmania, Australia 7001
Abstract
Grevillea (Proteaceae) is a woody Australian native genus with a high commercial value in the floriculture market. In this work the endogenous IAA and IBA were quantified in different plant parts using gas chromatography-mass spectrometry (GC-MS). Two Grevillea cultivars with different rooting ability were used, 'Poorinda Royal Mantle' (easy-to-root) and 'Coastal Dawn' (hard-to-root). There were no significant differences in the endogenous IAA or IBA levels between the easy and hard-to-root cultivars, either in the stem or in the leaves. A different distribution pattern of the applied (3H)-IBA was noted between 'Coastal Dawn' and 'Poorinda Royal Mantle'. The applied IBA in 'Coastal Dawn' moved to the leaf rather than the stem base, whereas in 'Poorinda Royal Mantle' it accumulated in the stem base. This study indicated the different mode of transport and partitioning of the applied IBA and the conversion of IBA to IAA in two Grevillea cultivars with different rooting abilities.
Key words: adventitious rooting, auxin transport, IAA, IBA
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