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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
8(4): 198-203, 2008

OPTIMIZATION OF FACTORS AFFECTING ORGANOGENESIS AND SOMATIC EMBRYOGENESIS OF ANTHURIUM ANDREANUM LIND. ‘TERA’

Ebrahim Beyramizade1, Pejman Azadi1,2, and Masahiro Mii2*

1 Department of Biotechnology, National Research Center of Ornamental Plants,
Mahallat, Iran.
2 Laboratory of Plant Cell Technology, Graduate School of Horticulture,
Chiba University, 648 Matsudo, Matsudo City, Chiba 271-8510, Japan,
*Fax: + 81 47 308 8720 *E-mail: miim@faculty.chiba-u.jp


Abstract
In vitro propagation of Anthurium has special importance for rapid clonal propagation and disease elimination. In this research, micropropagation of Anthurium andreanum ‘Tera’ was investigated through organogenesis and somatic embryogenesis. For organogenesis, best result for callus induction was obtained on half strength MS medium containing 0.08 mg l-1 2,4-D and 1 mg l-1 BA, whereas medium without phytohormones induced the highest number of shoots from the callus. Plantlets were successfully transferred to pots and grown in the greenhouse. For somatic embryogenesis, the highest percentage of somatic embryos was observed on medium containing 3 mg l-1 2,4-D and 0.33 mg l-1 kinetin. Conversion and maturation of embryos occurred on the same basal medium with 0.4 mg l-1 BA. After germination on phytohormone-free medium, plantlets were transferred to in vivo condition and grown to normal plants on a perlite bed.

Key words: Anthurium andreanum, callus, micropropagation, organogenesis, somatic embryogenesis



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