Micropropagation methods are currently being developed to improve the propagation for the production of Erysimum linifolium ‘Variegatum’ to meet consumer demand. For shoot proliferation, shoots tips and nodal explants were excised from stock plants and established on WPM medium supplemented with 0.88 μM BAP and 0.05 μM NAA. Shoots obtained from in vitro cultures were excised and maintained on three different media: (A1) modified MS and (A2) WPM each containing 6.65 μM BAP and 0.022 μM 2,4-D and (A3) MS with 0.88 μM BAP. Proliferated shoots were rooted on half strength MS medium supplemented with 0.049-49.2 μM IBA and one treatment where IBA was substituted by 3 g l^-1 activated charcoal (AC). Paclobutrazol (PAC) drenches were applied to plants weaned to the greenhouse at concentrations of 2.0-16.0 mg a.i. per 2 l compost. Shoot tip and nodal explants were readily established on WPM medium; however only 53.3% retained the variegated phenotype for both explant types. Proliferated shoots cultured on A3 produced the highest micropropagation rates (4.18) of explants, which retained the variegated phenotype. Explants cultured on MS supplemented with 0.49 μM IBA and MS where IBA was supplemented with 3 g l^-1 AC achieved 100% rooting; however, all explants cultured on media containing AC retained the variegated phenotype. Survival of explants on weaning was 100%. For greenhouse grown plants the application of PAC drenches significantly reduced both plant height and internode length even at the lowest level of 2.0 mg a.i. per 2 l compost.