ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
7(4): 184-189, 2007

IN VITRO PROPAGATION OF LITHODORA ROSMARINIFOLIA (TEN.)
JOHNST., A RARE ENDEMIC SICILIAN SHRUB WITH
POTENTIAL AS ORNAMENTAL PLANT

Giovanni Iapichino

Department of Agronomy - Horticultural and Floricultural Branch,
University of Palermo, Viale delle Scienze, 90128, Palermo, Italy,
Fax: + 39 91 6650259, E-mail: giapichino@unipa.it


Abstract
Lithodora rosmarinifolia is a rare shrub endemic to Sicily and its minor islands. Propagation of Lithodora rosmarinifolia by standard methods is difficult due to erratic seed production and low percentage of rooted cuttings. As the plant is suited for domestication, the possibility of establishing an efficient in vitro technique for propagation and conservation of this threatened species was investigated. Nodal segments from shoot tips were used as primary explants to establish in vitro culture. A 2 X 5 factorial experiment with five concentrations (0 to 17.74 μM) of BA and two concentrations (0 and 2.46 μM) of IBA was used to determine the optimum growth regulator combination for axillary shoot multiplication by nodal segments. In the subsequent experiment equal molar concentrations of three cytokinins [2-isopenthenyladenine (2iP), kinetin, and BA] in combination with equal molar concentrations of three auxins (IAA, NAA and IBA) were tested for ability to induce shoot proliferation. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog (MS) agar medium to which 4.44 μM BA and either 2.46 μM IBA or 2.46 μM IAA were added. In vitro rooting was efficiently accomplished in a medium containing ½ MS salts and vitamins, 20 g l-1 sucrose, 8 g l-1 agar and augmented with IAA at 0.11 μM. Rooted plants, transferred into soil, grew vigorously and flowered profusely during winter.

Key words: endemic, growth regulators, in vitro rooting, Lithodora rosmarinifolia, micropropagation





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