ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
7(2): 97-100, 2007

MICROPROPAGATION OF THE BAMBOO ORCHID
(ARUNDINA GRAMINIFOLIA (D. DON) HOCHR.)
THROUGH PROTOCORM-LIKE-BODIES USING NODE EXPLANTS

Kottackal Poulose Martin

Department of Biotechnology, University of Calicut, 673 635, India, E-mail: marttinet@yahoo.com


Abstract
High frequency micropropagation of Arundina graminifolia (D. Don.) Hochr. through protocorm-like bodies (PLBs) using node explants was established. Node explants derived from three-year-old field grown plants were cultured on half-strength MS medium with either 6.97 µM kinetin (Kn), or 15% coconut water (CW) or 13.3 µM BA favoured sprouting of the axillary bud. Subsequent culture of these emerged buds on medium having 44.4 µM BA facilitated formation of PLBs (a mean of 5.4) from the base. Transfer of PLBs to medium with the same level of BA (44.4 µM) PLBs favoured enhanced proliferation. The 6th subculture of PLBs on medium with 44.4 µM BA yielded > 100 PLBs. The PLB proliferation did not exhibit a decline up to 10th subculture. Conversion of PLBs to shoots or plantlets occurred at high rate (89%) upon transfer to half-strength MS medium containing 6.97 µM Kn. Half-strength MS medium with 1 g l-1 activated charcoal was effective for rooting, and the rooted plants exhibited 91% survival in field conditions.

Key words: Bamboo orchid, micropropagation, protocorm-like bodies



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