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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
7(2): 75-81, 2007

EFFECTS OF CHOPPER-LIGHT ON IN VITRO SHOOT CULTURES OF AMELANCHIER AND TILIA

Ina Pinker1* and Dieter Oellerich2

1Institute of Horticultural Sciences, Humboldt University of Berlin, Albrecht-Thaer-Weg 1,
D-14195 Berlin, Germany, *Fax: + 49-30-31471100, *E-mail: ina.pinker@agrar.hu-berlin.de
2Chopper Light GmbH, 7 Treuenbrietzener Str., 13439 Berlin, Germany, E-mail: DOellerich@aol.com


Abstract
The Chopper-Light-System produces intermittent light with common fluorescent tubes. The effect of Chopper-Light on multiplication and shoot development of in vitro shoot cultures of Amelanchier lamarckii and Tilia cordata was investigated at three photosynthetic active photon flux densities (PPFD): 22, 37 and 86 µmol m-2 s-1, applied in intervals of 100 ms light/100 ms dark (Chopper-Light) and with the continuous light (control) for a photoperiod of 16h daily. The shoot cultures were cultivated on modified MS-medium containing 30 g l-1 sucrose. Regardless of the lighting regime, shoot culture multiplication rates and fresh weight increased with PPFD value. There was no difference in multiplication and fresh weight of Amelanchier between Chopper-Light and control at a PPFD of 37 µmol m-2 s-1, however, at PPFD of 22 and 86 µmol m-2 s-1 multiplication rate and fresh weight in Chopper-Light was reduced compared to the control. In Tilia, no differences in multiplication rate and fresh weight could be found between the two lighting regimes. Generally, in Chopper-Light a higher number of longer shoots were formed.

Key words: intermittent light, photomixotrophic cultures, shoot multiplication



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