Propagation of Ornamental Plants
7(2): 62-67, 2007
IN VITRO CULTURE OF AN AFRICAN MULTIPURPOSE TREE SPECIES: ADANSONIA DIGITATA
Katsuaki Ishii1* and Sie Kambou2
1Forest Biotechnology Research Center, Forestry and Forest Products Research Institute (FFPRI), Matsunosato 1, Tsukuba, Ibaraki-ken, Japan 305-8687, *Tel.: + 81-298-829-8266, *Fax: + 81-829-873-1542, *E-mail: email@example.com
2Centre National de Semences Forestieres (CNSF), Burkina Faso
In vitro culture of Adansonia digitata (baobab), an important multipurpose tree, was performed. A. digitata seeds showed the best response to surface sterilization and were aseptically germinated and cultured in vitro. A high concentration of BAP suppressed root development in germination in vitro. All explants produced flourishing callus growth in vitro except the root segment. Callus formation was higher in half-strength Quoirin and Lepoivre (½LP) medium than in half-strengh Murashige and Skoog or half-strength Gamborg’s B5 medium. The cotyledonary portion was the best explant for callus formation. Shoots were obtained from nodal segments of in vitro germinated A. digitata cultured on ½LP medium containing 10 µM BAP. A rooted plantlet was regenerated on half-strength woody plant medium containing 3.5 µM IBA and 0.32 µM NAA. A regenerated plantlet was obtained in vitro for the first time.
Key words: Adansonia digitata, multipurpose trees, tissue culture