A novel method for Paphiopedilum delenatii Guillaumin micropropagation through shoots regenerated from elongated-plantlet-derived nodal segments was developed. Elongation of P. delenatii stem was induced under low photosynthetically active radiation (PAR). The elongated stems were then cut into nodal segments and cultured on modified MS medium supplemented with different concentrations of plant growth regulators for investigating their ability of shoot regeneration. Shoots were first initiated on modifited MS medium supplemented with 2.0 mg l^-1 BA after 15 days culture. However, these shoots were very small in size. Bigger shoots were obtained on medium supplemented with 1.5 mg l^-1 zeatin or 1.5 mg l^-1 TDZ with the regeneration rate of 58% and 75%, respectively. Healthy stem-node-derived plantlets were transferred onto a rooting medium before acclimatizing in the greenhouse. Elongation and stem node culture of P. delenatii will pave the way for further studies on the propagation of some other plants, especially recalcitrant species that have similar characteristics as Paphiopedilum.