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Propagation of Ornamental Plants
6(4): 201-204, 2006
EFFECTS OF 6-BENZYLAMINOPURINE, THIDIAZURON AND TYPE OF EXPLANT ON IN VITRO SHOOT DEVELOPMENT OF ACER PLATANOIDES L.
Leena Lindén1* and Anu Riikonen2
1Department of Applied Biology, P.O. Box 27, FI-00014 University of Helsinki, Finland,
*Fax.: +358-9-19158582, *E-mail: leena.linden@helsinki.fi
2Department of Forest Ecology, P.O. Box 27, FI-00014 University of Helsinki, Finland
Abstract
Apical and axillary bud explants from ca. 10-year-old Acer platanoides trees were cultured on Woody Plant Medium supplemented with different concentrations of TDZ and/or BAP. TDZ had a significant effect on shoot development, while positive effects of BAP were detected only in the absence of TDZ. Both apical and axillary bud explants proved suitable for micropropagation. The optimal TDZ concentration was between 0.01 and 0.1 µM. Axillary buds seemed to benefit from lower TDZ levels than apical bud explants. The overall shoot multiplication rate remained modest, and very little shoot elongation was achieved.
Key words: apical bud, axillary bud, micropropagation
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