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Propagation of Ornamental Plants
6(4): 180-186, 2006
ESTABLISHMENT OF CALLUS CULTURE WITH HIGH PLANT REGENERATION ABILITY FROM LEAF SEGMENTS OF LYSIONOTUS PAUCIFLORUS MAXIM.
Yuanxue Lu1, Toshinari Godo2*, Dong Poh Chin3, Masahiro Mii3, and Kaiyun Guan1
1Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Heilongtan, Kunming, Yunnan 650204, China
2Botanic Gardens of Toyama, 42 Kamikutsuwada, Fuchu-machi, Toyama 939-2713, Japan,
*Fax: +76-465-5923, *E-mail: godo@bgtym.org
33Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan
Abstract
Calli were induced from leaves of Lysionotus pauciflorus native to Yunnan, China, on gellan gum-solidified MS medium containing 2 μmol l-1 2,4-D. They showed 4 times proliferation in liquid medium of the same composition as callus induction after 4 weeks of culture. Shoots were induced from callus on gellan gum-solidified regeneration medium containing 32 μmol l-1 BA or 0.5 μmol l-1 zeatin. Also, numerous shoots were directly induced on leaf explants on medium containing low concentrations (0.25-8 μmol l-1) of NAA with or without 1 μmoll l-1 BA. Regenerated shoots rooted on half strength MS medium without any phytohormones.
Key words: adventitious shoot regeneration, callus, Lysionotus pauciflorus, micropropagation, plant growth regulator
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