Propagation of Ornamental Plants
5(2): 95-99, 2005
IN VITRO PROPAGATION OF TASMANNIA STIPITATA AC SMITH.
Sridevy Sriskandarajah¹* and Peter Goodwin²
¹*² Faculty of Agriculture, Food and Natural Resources, University of Sydney, NSW 2006,
Australia, *Fax: +45 3528 3400, *E-mail: email@example.com
¹* Present address: Plant and Soil Science Laboratory, Department of Agricultural Sciences,
The Royal Veterinary and Agricultural Universitysity, 40 Thorvaldsensvej,
1871 Frederiksberg C, Copenhagen, Denmark, Tel.: +45 3528 3409
Tasmannia stipitata is an aboriginal food plant and a potential contributor to the fledgling Australian bushfood industry. It is slow growing, and slow to propagate. Hence the following in vitro protocol has been developed. Excised shoots of field or glasshouse plants grew on Murashige and Skoog (1962) medium with half strength minerals and 3 µM kinetin. Growth was enhanced by the addition of 10% coconut water to the medium. About 8 lateral and adventitious buds in these shoots grew if small segments, of about 4 nodes, were taken, cut in half lengthwise, and incubated in medium lacking coconut milk, and supplemented with 6 µM CPPU or 30 µM BA, with CPPU giving the better result. The buds showed only slow growth on this medium, and for good elongation needed to be transferred to medium with 10% coconut milk and 10 µM 2iP plus 10 µM GA3. Approximately 60% rooting was obtained by planting 4 cm long shoots into a medium containing 15 µM IBA and 15 µM NAA for three days, and then transferring them to medium lacking growth regulators. It was possible to establish plants in soil at 75% recovery rate. The availability of a micropropagation method for Tasmannia is useful in expanding production of this potential bushfood.
Key words: micropropagation, pepperbush, tissue culture