Propagation of Ornamental Plants
5(2): 84-88, 2005
COST EFFECTIVE IN VITRO PROPAGATION OF MUSA ORNATA ROXB. THROUGH FLORAL TIP AXIS SEGMENT CULTURE
Kottackal Poulose Martin
Department of Biotechnology, University of Calicut, Kerala-673 635, India, Fax: +91 4942 400269, e-mail: firstname.lastname@example.org
A low cost micropropagation protocol for rapid propagation of an ornamentally important banana, Musa ornata Roxb. through floral tip segment culture was established. No significant differences in the development of shoots and roots were observed either on media prepared with commercial-grade sugar in tap water or with tissue culture-grade sucrose in double distilled water. The culture of non-sterilized floral tip segment explants on Murashige and Skoog (MS) medium with 17.8 µM N6-benzyladenine (BA) and 2.69 µM α-naphthaleneacetic acid (NAA) yielded the highest number, a mean of 4.7 shoots per explant within 100 days. The culture of isolated in vitro shoots on fresh medium having the same level of BA and NAA developed > 10 shoots. Multiplication of shoots occurred at a faster rate during subsequent cultures, but the shoots turned dwarf. The subsequent culture on medium supplemented with 6.66 µM BA reduced the dwarf nature of shoots. Half-strength MS medium with 2.69 µM NAA was superior for in vitro rooting. The shoots rooted on optimal rooting medium with 6% sugar exhibited slow growth, but were healthier with dark green leaves compared to 3% sugar supplemented medium. The plantlet size and sugar level in medium determined the plantlet survival in field conditions. Plantlets with a height of 5-7 cm from the optimal rooting medium containing 6% sugar resulted in 98% survival. Plantlets successfully established in field conditions were phenotypically similar to the mother plant. Use of tap water and commercial-grade sugar with high field survival allows en masse propagation of this ornamentally valuable plant with 70-80% reduction in production cost.
Key words: commercial-grade sugar, micropropagation, plantlet survival, tap water