A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentration of N6-benzyladenine (BA), N-benzyl-9-(2-tetrahydropyrenyl) adenine (BPA) and thidiazuron (TDZ), either singly or in combination with α-naphthaleneacetic acid (NAA), was used to determine the rate of shoot proliferation. N6-benzyladenine (BA) used at 0.5 mgl^-1 was the most effective in initiating shoot proliferation, producing an average of 23 microshoots per shoot-tip explant after 40 days of culture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 mg l^-1 indole-3-butyric acid (IBA). The plantlets were transferred to soil after acclimatization and showed 73% survival. These results represent a possible method for the mass production of plantlets through in vitro culture system of Zanthoxylum piperitum DC.