The effects of different concentrations of Indole-3-butyric acid (IBA), cutting lengths and cutting positions on the rooting of leafy stem cuttings of Pausinystalia johimbe K. Schum. were investigated in three experiments using non-mist propagators. A range of five IBA concentrations (0, 50, 100, 150 and 200 µg per 10 µl solution) was examined to investigate the auxin requirements for rooting stem cuttings. Highly significant differences (P <0.001) were recorded between IBA concentrations with respect to rooting percentages, values ranging from 60 ± 6.4% for 50 µg IBA to 95 ± 2.8% for 200 µg IBA per cutting. Compared to those treated with low IBA concentrations (0 and 50 µg IBA), cuttings treated with high IBA concentrations (100, 150 and 200 µg IBA) were most responsive (P <0.004) in terms of number of roots per cutting. Three stem cutting lengths namely 2, 4 and 6 cm were assessed for their influence on the rooting of leafy stem cuttings. Highly significant differences (P <0.004) were found in rooting percentage between cuttings of various lengths. At the end of the experiment, approximately 83 ± 4.9%, 78 ± 5.4% and 58 ± 6.4% rooting success was recorded, respectively from 6, 4 and 2 cm cutting stem length treatments. The mean number of roots per rooted cutting was significantly (P <0.05) affected by cutting stem lengths. The effects of three different node positions were also assessed. In contrast to stem length, cutting node position had no significant effect on final rooting percentage or mean number of roots, although the rooting percentage and mean number of roots tended to be high in apical nodes during the rooting period.