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Propagation of Ornamental Plants
3(1): 34-41, 2003
CURCUMA: STUDIES ON TISSUE CULTURE, POLLEN GERMINATION AND VIABILITY, HISTOLOGY AND FLOW CYTOMETRY
Waraporn Udomdee1, Seiichi Fukai2, Luckana Petpradap1 and Jaime A. Teixeira da Silva2*
1 Department of Horticulture, Maejo University, Chiang Mai, 50290, Thailand 2 Faculty of Agriculture, Kagawa University, Miki-cho, Ikenobe, 2393, Kagawa-ken, 761-0795, Japan, telfax: +81 (0)87 891 0747, e-mail: jaimetex@angelfire.com
Abstract
Curcuma is an extensive genus comprising of numerous species with ornamental, medicinal and aromatic importance. The biology of species within this genus is still poorly understood, and in an attempt to develop the knowledge base of various commercially important cultivars, studies on in vitro culture, pollen viability, germination and storage, ploidy, and histological analyses to determine floral and inflorescence ultra-structure and development were made. Curcuma alismatifolia "Lotus Pink" could be successfully tissue-cultured from rhizome explants on 2 mg l-1 BA-supplemented MS medium. Factors such as sucrose concentration, substrate culture base and incubation temperature affected germination efficiency of pollen, estimated by in vivo and in vitro methods while viability was tested by Aniline blue staining (98-99%). Pollen could not be stored for longer than 24 hours, with a subsequent exponential decrease in viability, and cryopreservation or other methods such low temperature storage or the use of organic solvents did not improve the viability of pollen following storage. Flow cytometric analyses showed genetic stability (i.e. no polysomaty) in material derived from any plant part or from different cultivars. A hypothetical model is suggested to explain the development of inflorescences and florets that develop in a predictable sequence of events.
Key words: development, flow cytometry, patumma, pollen storage, viability. *Author for correspondence
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