Continual large multiplication rates were obtained by subculturing shoot-tips of Jacaranda on B5 medium supplemented with a high concentration level of kinetin (100 µM) and solidified with 0.2 % (w/v) Gelrite. For elongation of proliferated shoots, reduction of the cytokinin level to 1 µM and addition of 0.5 % (w/v) activated charcoal to the medium was optimal. Maximal rooting frequencies were obtained on half-strength B5 medium supplemented with indole-3-butyric acid (IBA) alone, or in combination with naphthaleneacetic acid (NAA). Regenerated plantlets were transferred to vermiculite substratum and acclimatized successfully in a growth cabinet.