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Propagation of Ornamental Plants
2(1): 22-24, 2002
IN VITRO GERMINATION OF CYPRIPEDIUM DEBILE RCHB. f. IN RELATION TO CULTURE MEDIA AND SEED MATURITY
Masanori Tomita, Mina Tomita
Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo, Hirosaki, Aomori 036-8561, Japan, Tel: +81-172-39-3812, Fax: +81-172-39-3750,
e-mail: tomita@cc.hirosaki-u.ac.jp
Abstract
Seeds of Cypripedium debile at three different maturation stages were harvested, surface-sterilized and immediately inoculated on eleven different media. The fourteen weeks old immature seeds inoculated on inorganic salts of T medium with yeast extract showed the highest germination rate, while those at both earlier and later maturation stages showed a gradual decrease of germination rate.
Key words: Axenic culture, immature seeds, Cypripedium debile.
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