Propagation of Ornamental Plants
1(1): 41-45, 2001
ESTABLISHING MICROPROPAGATION CONDITIONS FOR THREE MAGNOLIA SPECIES
Aurelia Kamenická1, Andrej Kormut'ák2 and Maria Lanaková1
1Institute of Forest Ecology, Slovak Academy of Sciences, Vieska nad Zitavou, 951 52 Slepcany, Slovak Republic,
e-mail: aurelia@nr.sanet.sk
2Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademicka 2, P.O. Box 39A,
950 07 Nitra, Slovak Republic,
e-mail: nrgrkorm@savba.sk
Abstract
A micropropagation method for three Magnolia genotypes was established using nodal segments of adult plants.
The shoot cultures were grown in identical conditions on Standardi - Catalano medium supplemented with 2.22
µmol.dm -3 BAP and 1.07 µmol.dm -3 NAA. The growth and proliferation of genotype
Magnolia liliiflora were less affected as compared with the species Magnolia salicifolia and
Magnolia x soulangiana. The differences in the total biomass production of shoots among the species
tested were statistically significant. The highest number of shoot per explant (< 7) was obtained in
Magnolia salicifolia and Magnolia x soulangiana, whereas the greatest shoot length (< 16)
was obtained in Magnolia salicifolia. The mean length of produced shoots was lower in the species
Magnolia liliiflora. The method was proved to be convinient for the propagation of magnolia species
Magnolia x soulangiana, Magnolia liliiflora and Magnolia salicifolia, respectively.
Key words: Magnolia species, in vitro culture, regeneration, genetic stability.
Abbreviations: 6-benzylaminopurine (BAP), ? -naphthaleneacetic acid (NAA), 4-(3-indolyl) butyric acid (IBA)

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