A micropropagation method for three Magnolia genotypes was established using nodal segments of adult plants. The shoot cultures were grown in identical conditions on Standardi - Catalano medium supplemented with 2.22 µmol.dm^-3 BAP and 1.07 µmol.dm^-3 NAA. The growth and proliferation of genotype Magnolia liliiflora were less affected as compared with the species Magnolia salicifolia and Magnolia x soulangiana. The differences in the total biomass production of shoots among the species tested were statistically significant. The highest number of shoot per explant (< 7) was obtained in Magnolia salicifolia and Magnolia x soulangiana, whereas the greatest shoot length (< 16) was obtained in Magnolia salicifolia. The mean length of produced shoots was lower in the species Magnolia liliiflora. The method was proved to be convinient for the propagation of magnolia species Magnolia x soulangiana, Magnolia liliiflora and Magnolia salicifolia, respectively.

Abbreviations: 6-benzylaminopurine (BAP), ? -naphthaleneacetic acid (NAA), 4-(3-indolyl) butyric acid (IBA)