The influence of CO₂ concentrations (350 and 1200 µl.l^-1) on the growth of gerbera microcuttings was investigated with reference to light level (50 and 150 µmol.m^-2.s^-1 PPFD) and nutrient solution concentration (0.7, 1.4, 2.1 and 2.8 mS.cm^-1 EC). The best growth of cv. 'Ferrari' and 'Serena', cultivated on peat + perlite medium, was obtained at 1200 µl.l^-1 CO₂ and 150 µmol.m^-2.s^-1 PPFD. Carbon dioxide enrichment also accelerated the growth of 'Tamara', irrespective of PPFD level. The growth rate of Gerbera microcuttings cultivated in rockwool at CO₂ concentration was slow in all nutrient solution concentrations. The best growth of 'Serena' and 'Tamara' was observed in a CO₂ enriched atmosphere (1200 µl.l^-1) and at the highest level of electrical conductivity (EC 2.8 mS.cm^-1). The best growth of 'Ferrari' was at 1200 µl.l^-1 CO₂ and 2.1 mS.cm^-1 EC.